After the previous two studies found that ASU could provide symptomatic relief to individuals suffering from osteoarthritis, a third trial was conducted. In this third trial a much larger number of subjects participated, 260 total compared to the 150 in the prior two studies. The researchers conducted this trial using gold standard principles: multiple centers, use of a placebo, and both the subjects and the physicians were blind on who was taking ASU and who was taking the placebo.

The trial had some slight differences compared to the previous studies. In this trial, the group taking the ASU was split into ones taking 300mg of ASU or 600mg of ASU. The goal being to determine if more than 300mg ASU could give better results. Additionally, this trial just focused on individuals with knee pain. In total, everyone took the treatment for 3 months.

The results of the study are the following:

  • Increased joint function in both groups taking ASU compared to the group that only took a placebo. This functional change in joint function was seen after 60 days of ASU treatment. 
  • Decreased pain in both ASU groups compared to placebo that was noticeable after 30 days of ASU treatment. 
  • NSAID and analgesic use was decreased in the ASU groups compared to the people taking the placebo. 
  • The efficacy of ASU regardless of dose was consistently superior to people taking the placebo regardless of when and what endpoint was measured. 
  • No differences were observed between those taking 300mg of ASU and those taking 600mg of ASU. 

Overall, this study further corroborated the findings found in the two previous studies. ASU once again showed that it can improve pain and function in people suffering from knee pain. Additionally, this study demonstrated that the increased dose of 600mg of ASU was not better than 300mg of ASU. This is the reason the suggested daily dose of ASU is 300mg.  This was the third gold standard designed clinical trial that showed ASU can be an effective treatment for joint pain and for improving joint function.

Appelboom T, Schuermans J, Verbruggen G, Henrotin Y, Reginster JY. Symptoms modifying the effect of avocado/soybean unsaponifiables (ASU) in knee osteoarthritis. A double-blind, prospective, placebo-controlled study. Scand J Rheumatol. 2001;30(4):242-247.